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Research Technician, The Raghavan Lab

Dana-Farber Cancer Institute
United States, Massachusetts, Boston
November 05, 2022


Research Technician, The Raghavan Lab

Job Details

Job ID:
31060

Location:
450 Brookline Ave, Boston, MA 02215

Category:
Research Laboratory

Employment Type:
Full time

Work Location:
Onsite:Up to 1 day remote/wk

Overview

The Raghavan Laboratory at the Dana-Farber Cancer Institute (DFCI) and Harvard Medical School is seeking a highly motivated, collaborative and dedicated full-time research technician with a Bachelor's degree in Biology, Biomedical engineering, or related field to join the group. The Raghavan lab investigates how tumor cell states shape cancer cell behaviors, microenvironmental interactions, and therapeutic sensitivity and resistance, with a primary focus in pancreatic and biliary cancers. We apply a variety of techniques including single-cell sequencing of patient samples and cell models, patient-derived organoid culture, high throughput drug testing, and CRISPR engineering with the long-term goal of identifying new state- and context-specific cancer vulnerabilities. We work closely with clinicians in the Gastrointestinal Cancer Center at DFCI to combine clinical observations and patient-derived data with rigorous basic science investigation to identify impactful translational advances that ultimately improve patient care.

Responsibilities

We are seeking an enthusiastic, responsible, and motivated candidate with prior research experience in molecular and cellular biology and a minimum of a Bachelor's degree in Biology, Biochemistry, Bioengineering, or related disciplines. The ideal candidate will be detail- and goal-oriented, be able to work independently with appropriate supervision, and possess excellent organizational skills including the ability to handle a variety of tasks in a fast-paced environment. Strong interpersonal, communication, and analytical skills are also essential. Candidates with availability for a two-year commitment and interested in pursuing M.D., Ph.D., or M.D./Ph.D. training will benefit most from this laboratory experience.

This individual will work with a post-doctoral fellow or fellows using cutting-edge molecular biology, genomic, and pharmacologic techniques in a highly collaborative, stimulating, and multi-disciplinary research environment. This experience will enable the candidate to advance their skills in basic cancer biology with an eye toward translational applications for cancer care. The ability to work independently and to master a variety of cellular and molecular biology techniques is essential for this role, as are good organizational and communication skills, a desire to learn new scientific approaches, and the ability to function effectively within a team. All technicians will be authors on manuscripts published in the laboratory. Highly motivated and talented technicians will be given increasing responsibility and may eventually lead projects. She/he will also help with common lab maintenance tasks such as ordering supplies and aliquoting reagents. She/he will meet regularly with Dr. Raghavan to assess progress and receive mentorship and guidance.

Specific duties will include:



  • Tissue culture of cancer cell lines and patient-derived tumor organoid models
  • Processing of clinical samples for organoid development and sequencing
  • High-throughput drug testing assays
  • Single-cell RNA sequencing sample prep and data analysis
  • Chemical and genomic screens in cell line and organoid models
  • Transfections and viral-based CRISPR engineering of cellular models
  • Extraction and analysis of DNA and RNA, including PCR, quantitative PCR, RT-PCR
  • Protein assays including Western blotting and immunoprecipitation
  • DNA cloning and sequencing
  • Ordering and stocking of lab supplies and reagents
  • Preparation of reagents and media used in lab experiments
  • Operation and maintenance of standard lab equipment

To apply, please send a letter of interest, resume/CV, transcript, and list of 2-3 references to Srivatsan Raghavan ((see application details)).

Qualifications

Located in Boston and the surrounding communities, Dana-Farber Cancer Institute brings together world renowned clinicians, innovative researchers and dedicated professionals, allies in the common mission of conquering cancer and related diseases. Combining extremely talented people with the best technologies in a genuinely positive environment, we provide compassionate and comprehensive care to patients of all ages; we conduct research that advances treatment; we educate tomorrow's physician/researchers; we reach out to underserved members of our community; and we work with amazing partners, including other Harvard Medical School-affiliated hospitals. This position is offered at the Dana Farber Cancer Institute in partnership with the Broad Institute of Harvard and MIT, located in Cambridge, MA. DFCI and the Broad Institute bring together world-renowned clinical and technological expertise to study cancer and to develop novel therapeutic approaches.

Dana-Farber Cancer Institute is an equal opportunity employer and affirms the right of every qualified applicant to receive consideration for employment without regard to race, color, religion, sex, gender identity or expression, national origin, sexual orientation, genetic information, disability, age, ancestry, military service, protected veteran status, or other groups as protected by law.

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